4fte

X-ray diffraction
3.5Å resolution

Crystal structure of the D75N mutant capsid of Flock House virus

Released:
Entry authors: Speir JA, Chen Z, Reddy VS, Johnson JE

Function and Biology Details

Reaction catalysed:
Hydrolysis of an asparaginyl bond involved in the maturation of the structural protein of the virus, typically -Asn-|-Ala- or -Asn-|-Phe-.
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero 240-mer (preferred)
hetero tetramer
hetero icosamer
hetero 24-mer
hetero 80-mer
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Capsid protein alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 407 amino acids
Theoretical weight: 43.75 KDa
Source organism: Flock House virus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P12870 (Residues: 1-407; Coverage: 100%)
Gene name: alpha
Sequence domains: Peptidase A6 family
Structure domains: Jelly Rolls
Random cellular RNAs Chain: R
Molecule details ›
Chain: R
Length: 15 nucleotides
Theoretical weight: 4.57 KDa
Source organism: Spodoptera frugiperda

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: R3
Unit cell:
a: 325.6Å b: 325.6Å c: 770.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.239 not available
Expression system: Spodoptera frugiperda