4fmo

X-ray diffraction
3.04Å resolution

Structure of the C-terminal domain of the Saccharomyces cerevisiae MUTL alpha (MLH1/PMS1) heterodimer bound to a fragment of exo1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA mismatch repair protein MLH1 Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 33.24 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38920 (Residues: 485-769; Coverage: 37%)
Gene names: MLH1, PMS2, YM8520.16, YMR167W
Sequence domains: DNA mismatch repair protein Mlh1 C-terminus
DNA mismatch repair protein PMS1 Chain: B
Molecule details ›
Chain: B
Length: 240 amino acids
Theoretical weight: 27.95 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P14242 (Residues: 635-873; Coverage: 27%)
Gene names: N2317, PMS1, YNL082W
Sequence domains: MutL C terminal dimerisation domain
Exodeoxyribonuclease 1 Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 1.03 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P39875 (Residues: 443-450; Coverage: 1%)
Gene names: DHS1, EXO1, OR26.23, YOR033C

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 193.7Å b: 66.14Å c: 74.47Å
α: 90° β: 91.28° γ: 90°
R-values:
R R work R free
0.181 0.18 0.196
Expression systems:
  • Escherichia coli
  • Not provided