4fjo

X-ray diffraction
2.72Å resolution

Structure of the Rev1 CTD-Rev3/7-Pol kappa RIR complex

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 11.17 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q920Q2 (Residues: 1153-1249; Coverage: 8%)
Gene names: Rev1, Rev1l
Sequence domains: DNA repair protein REV1 C-terminal domain
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
DNA polymerase kappa Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.3 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9QUG2 (Residues: 565-574; Coverage: 1%)
Gene names: Dinb1, Polk
Mitotic spindle assembly checkpoint protein MAD2B Chain: C
Molecule details ›
Chain: C
Length: 210 amino acids
Theoretical weight: 24.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9D752 (Residues: 1-210; Coverage: 100%)
Gene names: Mad2b, Mad2l2, Rev7
Sequence domains: HORMA domain
Structure domains: HORMA domain
DNA polymerase zeta catalytic subunit Chain: D
Molecule details ›
Chain: D
Length: 30 amino acids
Theoretical weight: 3.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q61493 (Residues: 1865-1894; Coverage: 1%)
Gene names: Polz, Rev3l, Sez4

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P43212
Unit cell:
a: 145.727Å b: 145.727Å c: 70.799Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.236
Expression system: Escherichia coli BL21(DE3)