PDBe 4fhn

X-ray diffraction
6.99Å resolution

Nup37-Nup120 full-length complex from Schizosaccharomyces pombe

Released:
Primary publication:
Molecular basis for Nup37 and ELY5/ELYS recruitment to the nuclear pore complex.
Proc. Natl. Acad. Sci. U.S.A. 109 15241-6 (2012)
PMID: 22955883

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Uncharacterized WD repeat-containing protein C4F10.18 Chains: A, C
Molecule details ›
Chains: A, C
Length: 394 amino acids
Theoretical weight: 43.05 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O36030 (Residues: 1-391; Coverage: 100%)
Gene name: SPAC4F10.18
Sequence domains: WD domain, G-beta repeat
Nucleoporin nup120 Chains: B, D
Molecule details ›
Chains: B, D
Length: 1139 amino acids
Theoretical weight: 130.14 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43044 (Residues: 1-1136; Coverage: 100%)
Gene names: SPBC3B9.16c, nup120
Sequence domains: Nucleoporin Nup120/160
Glutamate dehydrogenase Chain: X
Molecule details ›
Chain: X
Length: 450 amino acids
Theoretical weight: 48.86 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XDW9 (Residues: 1-447; Coverage: 100%)
Gene names: ECpv15279_2279, ECs_2467, EFE38_25965, gdhA
Sequence domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6322
Unit cell:
a: 329.999Å b: 329.999Å c: 350.262Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.288 0.285 0.346
Expression system: Escherichia coli BL21(DE3)