› 4fhn

X-ray diffraction
6.99Å resolution

Nup37-Nup120 full-length complex from Schizosaccharomyces pombe

Released:
DOI: 10.2210/pdb4fhn/pdb
PDB 4fhn coloured by chain and viewed from the front.
PDB 4fhn coloured by chain and viewed from the side.
PDB 4fhn coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Molecular basis for Nup37 and ELY5/ELYS recruitment to the nuclear pore complex.
Bilokapic S, Schwartz TU
Proc Natl Acad Sci U S A 109 15241-6 (2012)
PMID: 22955883

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Uncharacterized WD repeat-containing protein C4F10.18 Chains: A, C
Molecule details ›
Chains: A, C
Length: 394 amino acids
Theoretical weight: 43.05 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O36030 (Residues: 1-391; Coverage: 100%)
Gene name: SPAC4F10.18
Sequence domains: WD domain, G-beta repeat
Nucleoporin nup120 Chains: B, D
Molecule details ›
Chains: B, D
Length: 1139 amino acids
Theoretical weight: 130.14 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43044 (Residues: 1-1136; Coverage: 100%)
Gene names: SPBC3B9.16c, nup120
Sequence domains: Nucleoporin Nup120/160
Glutamate dehydrogenase Chain: X
Molecule details ›
Chain: X
Length: 450 amino acids
Theoretical weight: 48.86 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XDW9 (Residues: 1-447; Coverage: 100%)
Gene names: E5F08_16190, ECs_2467, gdhA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6322
Unit cell:
a: 329.999Å b: 329.999Å c: 350.262Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.288 0.285 0.346
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The Nuclear Pore Complex as a Flexible and Dynamic Gate.
Knockenhauer et al. (2016)
2 more

4 mentions without citation

The Structure Inventory of the Nuclear Pore Complex.
Schwartz TU. (2016)
3 more