4f4w

X-ray diffraction
1.9Å resolution

Y-family DNA polymerase chimera Dbh-Dpo4-Dpo4 #1

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase IV Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 41.12 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q97W02 (Residues: 231-352; Coverage: 35%)
  • Canonical: Q4JB80 (Residues: 1-231; Coverage: 65%)
Gene names: SSO2448, Saci_0554, dbh, dpo4
Sequence domains:
Structure domains:
DNA (5'-D(*GP*GP*CP*AP*CP*TP*GP*AP*TP*CP*GP*GP*C)-3') Chains: E, P
Molecule details ›
Chains: E, P
Length: 13 nucleotides
Theoretical weight: 3.99 KDa
DNA (5'-D(*TP*TP*AP*CP*GP*GP*CP*CP*GP*AP*TP*CP*AP*GP*TP*GP*CP*C)-3') Chains: F, T
Molecule details ›
Chains: F, T
Length: 18 nucleotides
Theoretical weight: 5.49 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 52.82Å b: 99.655Å c: 101.625Å
α: 90° β: 90.16° γ: 90°
R-values:
R R work R free
0.187 0.186 0.219
Expression system: Escherichia coli