4f3t

X-ray diffraction
2.25Å resolution

Human Argonaute-2 - miR-20a complex

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-2 Chain: A
Molecule details ›
Chain: A
Length: 861 amino acids
Theoretical weight: 97.48 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UKV8 (Residues: 1-859; Coverage: 100%)
Gene names: AGO2, EIF2C2
Sequence domains:
Structure domains:
RNA (5'-R(P*UP*AP*AP*AP*GP*UP*GP*CP*UP*UP*AP*UP*AP*GP*UP*G*CP*AP*GP*G)-3') Chain: R
Molecule details ›
Chain: R
Length: 20 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 63.427Å b: 107.651Å c: 68.718Å
α: 90° β: 106.69° γ: 90°
R-values:
R R work R free
0.208 0.207 0.254
Expression systems:
  • Spodoptera frugiperda
  • Not provided