4f0z

X-ray diffraction
1.7Å resolution

Crystal Structure of Calcineurin in Complex with the Calcineurin-Inhibiting Domain of the African Swine Fever Virus Protein A238L

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 42.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08209 (Residues: 1-370; Coverage: 71%)
Gene names: CALNA, CNA, PPP3CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Calcineurin subunit B type 1 Chain: B
Molecule details ›
Chain: B
Length: 170 amino acids
Theoretical weight: 19.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63098 (Residues: 1-170; Coverage: 100%)
Gene names: CNA2, CNB, PPP3R1
Sequence domains:
Structure domains: EF-hand
IkB-like protein Chain: C
Molecule details ›
Chain: C
Length: 43 amino acids
Theoretical weight: 5.11 KDa
Source organism: African swine fever virus Malawi LIL 20/1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O36972 (Residues: 200-239; Coverage: 17%)
Gene names: 5EL, A238L, Mal-047

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 72.691Å b: 48.976Å c: 82.442Å
α: 90° β: 104.37° γ: 90°
R-values:
R R work R free
0.158 0.157 0.178
Expression system: Escherichia coli BL21(DE3)