4erq

X-ray diffraction
1.91Å resolution

X-ray structure of WDR5-MLL2 Win motif peptide binary complex

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 5 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 312 amino acids
Theoretical weight: 34.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 23-334; Coverage: 93%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone-lysine N-methyltransferase 2D Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 14 amino acids
Theoretical weight: 1.5 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O14686 (Residues: 5333-5346; Coverage: 0%)
Gene names: ALR, KMT2D, MLL2, MLL4

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 139.78Å b: 80.79Å c: 88.12Å
α: 90° β: 90.197° γ: 90°
R-values:
R R work R free
0.167 0.165 0.2
Expression systems:
  • Escherichia coli
  • Not provided