› 4emo

X-ray diffraction
2Å resolution

Crystal structure of the PH domain of SHARPIN

Released:
DOI: 10.2210/pdb4emo/pdb
PDB entry 4emo coloured by chain, front view.
PDB entry 4emo coloured by chain, side view.
PDB entry 4emo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of SHARPIN, a subunit of a large multi-protein E3 ubiquitin ligase, reveals a novel dimerization function for the pleckstrin homology superfold.
Stieglitz B, Haire LF, Dikic I, Rittinger K
J Biol Chem 287 20823-9 (2012)
PMID: 22549881

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sharpin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 129 amino acids
Theoretical weight: 13.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H0F6 (Residues: 1-127; Coverage: 33%)
Gene names: PSEC0216, SHARPIN, SIPL1
Sequence domains: Sharpin PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P43212
Unit cell:
a: 61.55Å b: 61.55Å c: 222.813Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Linear ubiquitination-mediated NF-κB regulation and its related disorders.
Tokunaga F. (2013)
2 more

11 mentions without citation

Linear ubiquitin chains: enzymes, mechanisms and biology.
Rittinger et al. (2017)
10 more