PDBe 4emi

X-ray diffraction
1.81Å resolution

Toluene dioxygenase reductase in reduced state in complex with NAD+


Function and Biology Details

Reaction catalysed:
2 reduced [2Fe-2S] ferredoxin + NAD(+) + H(+) = 2 oxidized [2Fe-2S] ferredoxin + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Toluene 1,2-dioxygenase system ferredoxin--NAD(+) reductase component Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 42.98 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
  • Canonical: A5W4E9 (Residues: 1-410; Coverage: 100%)
Gene names: Pput_2878, todA
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand NAD 1 x NAD

Cofactor: Ligand FAD 1 x FAD
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P41212
Unit cell:
a: 78.434Å b: 78.434Å c: 158.668Å
α: 90° β: 90° γ: 90°
R R work R free
0.185 0.184 0.215
Expression system: Escherichia coli