› 4e29

X-ray diffraction
1.6Å resolution

Periplasmic domain of the chimeric WzzB chain length regulator protein

Released:
DOI: 10.2210/pdb4e29/pdb
PDB entry 4e29 coloured by chain, front view.
PDB entry 4e29 coloured by chain, side view.
PDB entry 4e29 coloured by chain, top view.
Source organisms:
Primary publication:
Structural characterization of closely related O-antigen lipopolysaccharide (LPS) chain length regulators.
Kalynych S, Yao D, Magee J, Cygler M
J Biol Chem 287 15696-705 (2012)
PMID: 22437828

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chain length determinant protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 28.32 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37792 (Residues: 54-200; Coverage: 45%)
  • Canonical: Q04866 (Residues: 201-293; Coverage: 28%)
Gene names: S2210, SF2089, STM2079, cld, rol, wzzB
Sequence domains: Chain length determinant protein
Structure domains: FepE-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I422
Unit cell:
a: 115.757Å b: 115.757Å c: 210.885Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Synthase-dependent exopolysaccharide secretion in Gram-negative bacteria.
Whitney et al. (2013)
5 more

5 mentions without citation

Current Progress in the Structural and Biochemical Characterization of Proteins Involved in the Assembly of Lipopolysaccharide.
Bohl et al. (2018)
4 more