› 4dwx

X-ray diffraction
1.8Å resolution

Crystal Structure of a Family GH-19 Chitinase from rye seeds

Released:
DOI: 10.2210/pdb4dwx/pdb
PDB entry 4dwx coloured by chain, front view.
PDB entry 4dwx coloured by chain, side view.
PDB entry 4dwx coloured by chain, top view.
Source organism: Secale cereale
Primary publication:
Crystal structure and chitin oligosaccharide-binding mode of a 'loopful' family GH19 chitinase from rye, Secale cereale, seeds.
Ohnuma T, Numata T, Osawa T, Inanaga H, Okazaki Y, Shinya S, Kondo K, Fukuda T, Fukamizo T
FEBS J 279 3639-3651 (2012)
PMID: 22831795

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Basic endochitinase C Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 26.26 KDa
Source organism: Secale cereale
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FRV0 (Residues: 24-266; Coverage: 100%)
Gene name: rscc
Sequence domains: Chitinase class I
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MES 2 x MES
Ligand SO4 3 x SO4
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 64.914Å b: 84.684Å c: 108.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.204
Expression system: Escherichia coli

Quick links

Citations

4 review citations

From bacteria to human: a journey into the world of chitinases.
Adrangi et al. (2013)
3 more

8 mentions without citation

Structure of potassium channels.
Kuang et al. (2015)
7 more