4dep

X-ray diffraction
3.1Å resolution

Structure of the IL-1b signaling complex

Released:
Source organism: Homo sapiens
Primary publication:
Structure of the activating IL-1 receptor signaling complex.
Nat. Struct. Mol. Biol. 19 455-7 (2012)
PMID: 22426547

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-1 beta Chains: A, D
Molecule details ›
Chains: A, D
Length: 158 amino acids
Theoretical weight: 17.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01584 (Residues: 117-269; Coverage: 57%)
Gene names: IL1B, IL1F2
Sequence domains: Interleukin-1 / 18
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
Interleukin-1 receptor type 1, soluble form Chains: B, E
Molecule details ›
Chains: B, E
Length: 321 amino acids
Theoretical weight: 36.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P14778 (Residues: 18-336; Coverage: 58%)
Gene names: IL1R, IL1R1, IL1RA, IL1RT1
Sequence domains:
Structure domains: Immunoglobulins
Interleukin-1 receptor accessory protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 349 amino acids
Theoretical weight: 40.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NPH3 (Residues: 21-367; Coverage: 63%)
Gene names: C3orf13, IL1R3, IL1RAP
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 105.99Å b: 65.9Å c: 163.4Å
α: 90° β: 90.35° γ: 90°
R-values:
R R work R free
0.213 0.21 0.277
Expression systems:
  • Escherichia coli
  • Homo sapiens