› 4den

X-ray diffraction
1.6Å resolution

Structural insightsinto potent, specific anti-HIV property of actinohivin; Crystal structure of actinohivin in complex with alpha(1-2) mannobiose moiety of high-mannose type glycan of gp120

Released:
DOI: 10.2210/pdb4den/pdb
PDB entry 4den coloured by chain, front view.
PDB entry 4den coloured by chain, side view.
PDB entry 4den coloured by chain, top view.
Source organism: actinomycete K97-0003
Primary publication:
Structural insights into the specific anti-HIV property of actinohivin: structure of its complex with the α(1-2)mannobiose moiety of gp120.
Hoque MM, Suzuki K, Tsunoda M, Jiang J, Zhang F, Takahashi A, Ohbayashi N, Zhang X, Tanaka H, Omura S, Takénaka A
Acta Crystallogr D Biol Crystallogr 68 1671-9 (2012)
PMID: 23151632

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Actinohivin Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.53 KDa
Source organism: actinomycete K97-0003
UniProt:
  • Canonical: Q9KWN0 (Residues: 47-160; Coverage: 85%)
Gene name: ath
Sequence domains: Ricin-type beta-trefoil lectin domain
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
1 bound ligand:
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P213
Unit cell:
a: 56.217Å b: 56.217Å c: 56.217Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.145 0.202

Quick links

Citations

2 review citations

Advances in molecular engineering of carbohydrate-binding modules.
Armenta et al. (2017)
1 more

5 mentions without citation

The geometry of Niggli reduction: SAUC - search of alternative unit cells.
McGill et al. (2014)
4 more