4dck

X-ray diffraction
2.2Å resolution

Crystal structure of the C-terminus of voltage-gated sodium channel in complex with FGF13 and CaM

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero nonamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium channel protein type 5 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14524 (Residues: 1773-1940; Coverage: 8%)
Gene name: SCN5A
Structure domains:
Calmodulin-2 Chain: B
Molecule details ›
Chain: B
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP24 (Residues: 1-149; Coverage: 100%)
Gene names: CALM2, CAM2, CAMB
Sequence domains: EF-hand domain pair
Fibroblast growth factor 13 Chain: C
Molecule details ›
Chain: C
Length: 192 amino acids
Theoretical weight: 21.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92913 (Residues: 63-245; Coverage: 75%)
Gene names: FGF13, FHF2
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID, APS BEAMLINE 24-ID-C
Spacegroup: P213
Unit cell:
a: 126.041Å b: 126.041Å c: 126.041Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.227
Expression system: Escherichia coli