4dbg

X-ray diffraction
2.71Å resolution

Crystal structure of HOIL-1L-UBL complexed with a HOIP-UBA derivative

Released:

Function and Biology Details

Reaction catalysed:
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RanBP-type and C3HC4-type zinc finger-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BYM8 (Residues: 37-137; Coverage: 20%)
Gene names: C20orf18, RBCK1, RNF54, UBCE7IP3, XAP3, XAP4
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
E3 ubiquitin-protein ligase RNF31 Chain: B
Molecule details ›
Chain: B
Length: 162 amino acids
Theoretical weight: 18.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96EP0 (Residues: 480-636; Coverage: 15%)
Gene names: RNF31, ZIBRA
Sequence domains: HOIP UBA domain pair
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P3121
Unit cell:
a: 64.973Å b: 64.973Å c: 161.291Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.218 0.252
Expression system: Escherichia coli