PDBe 4day

X-ray diffraction
3.3Å resolution

Crystal structure of the RMI core complex with MM2 peptide from FANCM

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RecQ-mediated genome instability protein 1 Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 17.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H9A7 (Residues: 473-625; Coverage: 25%)
Gene names: C9orf76, RMI1
Sequence domains: Recq-mediated genome instability protein 1, C-terminal OB-fold
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
RecQ-mediated genome instability protein 2 Chain: B
Molecule details ›
Chain: B
Length: 150 amino acids
Theoretical weight: 16.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96E14 (Residues: 1-147; Coverage: 100%)
Gene names: C16orf75, RMI2
Sequence domains: RecQ-mediated genome instability protein 2
Structure domains: Nucleic acid-binding proteins
Fanconi anemia group M protein Chain: C
Molecule details ›
Chain: C
Length: 37 amino acids
Theoretical weight: 4.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IYD8 (Residues: 1218-1251; Coverage: 2%)
Gene names: FANCM, KIAA1596

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I212121
Unit cell:
a: 75.312Å b: 96.44Å c: 99.025Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.216 0.325
Expression system: Escherichia coli