4d1q

X-ray diffraction
3.4Å resolution

Hermes transposase bound to its terminal inverted repeat

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
BED-type domain-containing protein Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 536 amino acids
Theoretical weight: 61.32 KDa
Source organism: Musca domestica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q25442 (Residues: 79-612; Coverage: 87%)
Sequence domains:
Structure domains: Zinc finger, BED domain-containing
TERMINAL INVERTED REPEAT Chains: C, E, I, K
Molecule details ›
Chains: C, E, I, K
Length: 15 nucleotides
Theoretical weight: 4.61 KDa
Source organism: Musca domestica
Expression system: Not provided
TERMINAL INVERTED REPEAT Chains: D, F, J, L
Molecule details ›
Chains: D, F, J, L
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Musca domestica
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3221
Unit cell:
a: 265.15Å b: 265.15Å c: 157.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.254
Expression systems:
  • Escherichia coli
  • Not provided