› 4d1k

Electron Microscopy
9.4Å resolution

Cryo-electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly.

Released:
DOI: 10.2210/pdb4d1k/pdb
PDB entry 4d1k coloured by chain, front view.
PDB entry 4d1k coloured by chain, side view.
PDB entry 4d1k coloured by chain, top view.
Source organism: Human immunodeficiency virus 1
Primary publication:
Cryo-electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly.
Bharat TA, Castillo Menendez LR, Hagen WJ, Lux V, Igonet S, Schorb M, Schur FK, Kräusslich HG, Briggs JA
Proc Natl Acad Sci U S A 111 8233-8 (2014)
PMID: 24843179
Related structures: EMD-2638

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retroviral nucleocapsid Gag protein p24 C-terminal domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 219 amino acids
Theoretical weight: 24.42 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5D0H3 (Residues: 1-219; Coverage: 95%)
Gene name: gag
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.4Å
Relevant EMDB volumes: EMD-2638
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

How HIV-1 Gag assembles in cells: Putting together pieces of the puzzle.
Lingappa et al. (2014)
18 more

3 mentions without citation

Cryo-electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly.
Bharat et al. (2014)
2 more