4czz

X-ray diffraction
3Å resolution

Histone demethylase LSD1(KDM1A)-CoREST3 Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysine-specific histone demethylase 1A Chain: A
Molecule details ›
Chain: A
Length: 872 amino acids
Theoretical weight: 94.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O60341 (Residues: 1-173, 174-852; Coverage: 100%)
  • Best match: O60341-2 (Residues: 1-876)
Gene names: AOF2, KDM1, KDM1A, KIAA0601, LSD1
Sequence domains:
Structure domains:
REST corepressor 3 Chain: B
Molecule details ›
Chain: B
Length: 553 amino acids
Theoretical weight: 61.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9P2K3 (Residues: 1-495; Coverage: 100%)
  • Best match: Q9P2K3-3 (Residues: 1-553)
Gene names: KIAA1343, RCOR3
Sequence domains:
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I222
Unit cell:
a: 118.04Å b: 177.44Å c: 235.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.236
Expression system: Escherichia coli BL21