X-ray diffraction
3.16Å resolution

Structure of human iNOS heme domain in complex with (R)-6-(3-AMINO-2-(5-(2-(6-AMINO-4- METHYLPYRIDIN-2-YL)ETHYL)PYRIDIN-3-YL)PROPYL)-4- METHYLPYRIDIN-2-AMINE


Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Nitric oxide synthase, inducible Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 49.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P35228 (Residues: 74-504; Coverage: 37%)
Gene names: NOS2, NOS2A
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments

Cofactor: Ligand HEM 4 x HEM

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P43212
Unit cell:
a: 189.223Å b: 189.223Å c: 232.773Å
α: 90° β: 90° γ: 90°
R R work R free
0.176 0.174 0.216
Expression system: Escherichia coli BL21(DE3)