4ct4

X-ray diffraction
2.3Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CCR4-NOT transcription complex subunit 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 258 amino acids
Theoretical weight: 29.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5YKK6 (Residues: 1063-1314; Coverage: 11%)
Gene names: AD-005, CDC39, CNOT1, KIAA1007, NOT1
Sequence domains: CCR4-NOT transcription complex subunit 1 CAF1-binding domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Probable ATP-dependent RNA helicase DDX6 Chains: B, D
Molecule details ›
Chains: B, D
Length: 378 amino acids
Theoretical weight: 43.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26196 (Residues: 95-469; Coverage: 78%)
Gene names: DDX6, HLR2, RCK
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 71.531Å b: 120.014Å c: 78.469Å
α: 90° β: 104.01° γ: 90°
R-values:
R R work R free
0.169 0.166 0.22
Expression system: Escherichia coli BL21(DE3)