PDBe 4cs2

X-ray diffraction
1.9Å resolution

Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in its apo form

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrrolysine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.74 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PWY1 (Residues: 188-454; Coverage: 59%)
Gene names: MM_1445, pylS
Sequence domains: tRNA synthetases class II core domain (F)
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 102.179Å b: 43.492Å c: 63.266Å
α: 90° β: 99.96° γ: 90°
R-values:
R R work R free
0.18 0.178 0.216
Expression system: Escherichia coli