4crn

Electron Microscopy
9.1Å resolution

Cryo-EM of a pretermination complex with eRF1 and eRF3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic peptide chain release factor GTP-binding subunit Chain: P
Molecule details ›
Chain: P
Length: 430 amino acids
Theoretical weight: 47.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P05453 (Residues: 256-685; Coverage: 63%)
Gene names: GST1, PNM2, SAL3, SUF12, SUP2, SUP35, YD9395.05, YDR172W
Sequence domains:
Eukaryotic peptide chain release factor subunit 1 Chain: X
Molecule details ›
Chain: X
Length: 437 amino acids
Theoretical weight: 49.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P12385 (Residues: 1-437; Coverage: 100%)
Gene names: SAL4, SUP1, SUP45, YBR1120, YBR143C
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.1Å
Relevant EMDB volumes: EMD-2597
Expression system: Escherichia coli