4cn2

X-ray diffraction
2.07Å resolution

Crystal Structure of the Human Retinoid X Receptor DNA-Binding Domain Bound to the Human Ramp2 Response Element

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Retinoic acid receptor RXR-alpha Chains: C, D
Molecule details ›
Chains: C, D
Length: 87 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19793 (Residues: 130-212; Coverage: 18%)
Gene names: NR2B1, RXRA
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
5'-D(*TP*GP*AP*GP*TP*TP*CP*AP*AP*GP*GP*GP*TP*DC *AP*AP*TP)-3' Chain: A
Molecule details ›
Chain: A
Length: 17 nucleotides
Theoretical weight: 5.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*AP*TP*TP*GP*AP*CP*CP*CP*TP*TP*GP*AP*AP*DC *TP*CP*AP)-3' Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.15 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 113.234Å b: 43.952Å c: 63.316Å
α: 90° β: 106.02° γ: 90°
R-values:
R R work R free
0.167 0.165 0.214
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided