4ce4

Electron Microscopy
4.9Å resolution

39S large subunit of the porcine mitochondrial ribosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 38-mer (preferred)
Entry contents:
35 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (37 distinct):
Mitochondrial ribosomal protein L27 Chain: 0
Molecule details ›
Chain: 0
Length: 148 amino acids
Theoretical weight: 15.94 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1RT79 (Residues: 1-148; Coverage: 100%)
Gene name: MRPL27
Sequence domains: Ribosomal L27 protein
ICT1 Chain: 1
Molecule details ›
Chain: 1
Length: 256 amino acids
Theoretical weight: 30.15 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: A0A0R4J8D5 (Residues: 71-97; Coverage: 17%)
Gene name: MRPL18
Mitochondrial ribosomal protein L47 Chain: 2
Molecule details ›
Chain: 2
Length: 252 amino acids
Theoretical weight: 30.09 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1SGC7 (Residues: 1-252; Coverage: 100%)
Gene name: MRPL47
Sequence domains: Mitochondrial 39-S ribosomal protein L47 (MRP-L47)
39S ribosomal protein L30, mitochondrial Chain: 3
Molecule details ›
Chain: 3
Length: 161 amino acids
Theoretical weight: 18.59 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1STE5 (Residues: 8-161; Coverage: 96%)
Gene name: MRPL30
Sequence domains: Ribosomal protein L30p/L7e
MRPL32 Chain: 5
Molecule details ›
Chain: 5
Length: 146 amino acids
Theoretical weight: 15.93 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: I3LTC4 (Residues: 20-145; Coverage: 61%)
Gene name: MRPL32
MRPL33 Chain: 6
Molecule details ›
Chain: 6
Length: 65 amino acids
Theoretical weight: 7.51 KDa
Source organism: Sus scrofa domesticus
39S ribosomal protein L34, mitochondrial Chain: 7
Molecule details ›
Chain: 7
Length: 95 amino acids
Theoretical weight: 10.87 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: W5IDC1 (Residues: 1-95; Coverage: 100%)
Gene name: MRPL34
Sequence domains: Ribosomal protein L34
39S ribosomal protein L35, mitochondrial Chain: 8
Molecule details ›
Chain: 8
Length: 188 amino acids
Theoretical weight: 21.55 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1SVC5 (Residues: 1-188; Coverage: 100%)
Gene name: MRPL35
Sequence domains: Ribosomal protein L35
MRPL36 Chain: 9
Molecule details ›
Chain: 9
Length: 100 amino acids
Theoretical weight: 10.84 KDa
Source organism: Sus scrofa domesticus
Ribosomal_L2 domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 306 amino acids
Theoretical weight: 33.27 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1RRN2 (Residues: 1-216; Coverage: 96%)
Gene name: MRPL2
Sequence domains: Ribosomal Proteins L2, RNA binding domain
MRPL3 Chain: E
Molecule details ›
Chain: E
Length: 399 amino acids
Theoretical weight: 43.71 KDa
Source organism: Sus scrofa domesticus
Mitochondrial ribosomal protein L4 Chain: F
Molecule details ›
Chain: F
Length: 224 amino acids
Theoretical weight: 24.31 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1S3J8 (Residues: 94-289; Coverage: 63%)
Gene name: MRPL4
Sequence domains: Ribosomal protein L4/L1 family
39S ribosomal protein L9, mitochondrial Chain: I
Molecule details ›
Chain: I
Length: 268 amino acids
Theoretical weight: 30.37 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: I3LR45 (Residues: 1-268; Coverage: 100%)
Gene name: MRPL9
Sequence domains: Ribosomal protein L9, N-terminal domain
MRPL13 Chain: N
Molecule details ›
Chain: N
Length: 178 amino acids
Theoretical weight: 20.63 KDa
Source organism: Sus scrofa domesticus
Mitochondrial ribosomal protein L14 Chain: O
Molecule details ›
Chain: O
Length: 145 amino acids
Theoretical weight: 15.96 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1RQV7 (Residues: 49-193; Coverage: 75%)
Gene name: MRPL14
Sequence domains: Ribosomal protein L14p/L23e
39S ribosomal protein L15, mitochondrial Chain: P
Molecule details ›
Chain: P
Length: 288 amino acids
Theoretical weight: 32.47 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1RSH0 (Residues: 22-140, 141-293; Coverage: 93%)
Gene name: MRPL15
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
39S ribosomal protein L16, mitochondrial Chain: Q
Molecule details ›
Chain: Q
Length: 208 amino acids
Theoretical weight: 22.75 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1RI89 (Residues: 1-191; Coverage: 76%)
Gene name: MRPL16
Sequence domains: Ribosomal protein L16p/L10e
39S ribosomal protein L17, mitochondrial Chain: R
Molecule details ›
Chain: R
Length: 169 amino acids
Theoretical weight: 19.27 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1RMQ4 (Residues: 1-169; Coverage: 100%)
Gene name: MRPL17
Sequence domains: Ribosomal protein L17
ICT1 Chain: S
Molecule details ›
Chain: S
Length: 180 amino acids
Theoretical weight: 20.58 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: A0A0R4J8D5 (Residues: 1-180; Coverage: 100%)
Gene name: MRPL18
Sequence domains: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
39S ribosomal protein L19, mitochondrial Chain: T
Molecule details ›
Chain: T
Length: 292 amino acids
Theoretical weight: 33.44 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: I3LNJ0 (Residues: 12-303; Coverage: 96%)
Gene name: MRPL19
Sequence domains: Ribosomal protein L19
MRPL20 Chain: U
Molecule details ›
Chain: U
Length: 132 amino acids
Theoretical weight: 15.56 KDa
Source organism: Sus scrofa domesticus
39S ribosomal protein L21, mitochondrial isoform d Chain: V
Molecule details ›
Chain: V
Length: 207 amino acids
Theoretical weight: 22.89 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1RY70 (Residues: 1-203; Coverage: 97%)
Sequence domains: Ribosomal prokaryotic L21 protein
39S ribosomal protein L22, mitochondrial Chain: W
Molecule details ›
Chain: W
Length: 134 amino acids
Theoretical weight: 15.07 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: W5IDB8 (Residues: 1-110; Coverage: 82%)
Sequence domains: Ribosomal protein L22p/L17e
Mrpl23 Chain: X
Molecule details ›
Chain: X
Length: 87 amino acids
Theoretical weight: 9.81 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: W5IDB9 (Residues: 15-82; Coverage: 78%)
KOW domain-containing protein Chain: Y
Molecule details ›
Chain: Y
Length: 216 amino acids
Theoretical weight: 24.89 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1RHJ1 (Residues: 1-216; Coverage: 100%)
Gene name: MRPL24
Sequence domains:
39S ribosomal protein L38, mitochondrial Chain: b
Molecule details ›
Chain: b
Length: 380 amino acids
Theoretical weight: 44.66 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1RW03 (Residues: 1-380; Coverage: 100%)
Gene name: MRPL38
Sequence domains: Phosphatidylethanolamine-binding protein
TGS domain-containing protein Chain: c
Molecule details ›
Chain: c
Length: 301 amino acids
Theoretical weight: 33.83 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1SHP6 (Residues: 66-303, 304-318; Coverage: 80%)
Gene name: MRPL39
DRBM domain-containing protein Chain: h
Molecule details ›
Chain: h
Length: 298 amino acids
Theoretical weight: 32.64 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: F1SR64 (Residues: 67-332; Coverage: 80%)
Gene name: MRPL44
Tim44 domain-containing protein Chain: i
Molecule details ›
Chain: i
Length: 312 amino acids
Theoretical weight: 36.12 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: I3LGM5 (Residues: 1-306; Coverage: 100%)
Gene name: MRPL45
Sequence domains: Tim44-like domain
ICT1 Chain: l
Molecule details ›
Chain: l
Length: 166 amino acids
Theoretical weight: 18.96 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: A0A0R4J8D5 (Residues: 146-150, 151-163; Coverage: 11%)
Gene name: MRPL18
MRPL52 Chain: o
Molecule details ›
Chain: o
Length: 56 amino acids
Theoretical weight: 4.78 KDa
Source organism: Sus scrofa domesticus
RF_PROK_I domain-containing protein Chain: u
Molecule details ›
Chain: u
Length: 205 amino acids
Theoretical weight: 23.55 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: W5IDC0 (Residues: 1-205; Coverage: 100%)
Sequence domains: RF-1 domain
UNASSIGNED HELICES Chains: v, w
Molecule details ›
Chains: v, w
Length: 91 amino acids
Theoretical weight: 7.76 KDa
Source organism: Sus scrofa domesticus
THIOREDOXIN FOLD Chain: x
Molecule details ›
Chain: x
Length: 85 amino acids
Theoretical weight: 7.25 KDa
Source organism: Sus scrofa domesticus
UNASSIGNED HELICES Chain: z
Molecule details ›
Chain: z
Length: 426 amino acids
Theoretical weight: 36.27 KDa
Source organism: Sus scrofa domesticus
16S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1570 nucleotides
Theoretical weight: 501.42 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
UNASSIGNED RNA Chain: B
Molecule details ›
Chain: B
Length: 29 nucleotides
Theoretical weight: 5.64 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.9Å
Relevant EMDB volumes: EMD-2490