4cbl

X-ray diffraction
3.05Å resolution

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease NS3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 509 amino acids
Theoretical weight: 57.22 KDa
Source organism: Classical swine fever virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P19712 (Residues: 1792-2280; Coverage: 13%)
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 69.91Å b: 156.84Å c: 99.4Å
α: 90° β: 97.21° γ: 90°
R-values:
R R work R free
0.196 0.194 0.226
Expression system: Escherichia coli BL21