› 4c7a

X-ray diffraction
2.3Å resolution

Crystal structure of the Smoothened CRD, selenomethionine-labeled

Released:
DOI: 10.2210/pdb4c7a/pdb
PDB entry 4c7a coloured by chain, front view.
PDB entry 4c7a coloured by chain, side view.
PDB entry 4c7a coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling.
Nachtergaele S, Whalen DM, Mydock LK, Zhao Z, Malinauskas T, Krishnan K, Ingham PW, Covey DF, Siebold C, Rohatgi R
Elife 2 e01340 (2013)
PMID: 24171105

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177783 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein smoothened Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 22.33 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5RH73 (Residues: 28-210; Coverage: 23%)
Gene names: smo, smu
Sequence domains: Fz domain
Structure domains: Frizzled cysteine-rich domain

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand NA 3 x NA
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P43212
Unit cell:
a: 68.13Å b: 68.13Å c: 92.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

The Adult Ventricular-Subventricular Zone (V-SVZ) and Olfactory Bulb (OB) Neurogenesis.
Lim et al. (2016)
39 more

1 mention without citation

Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling.
Nachtergaele et al. (2013)