X-ray diffraction
2.1Å resolution

Structure of the Mycobacterium tuberculosis APS kinase CysC in complex with ADP and APS


Function and Biology Details

Reactions catalysed:
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
ATP + sulfate = diphosphate + adenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Bifunctional enzyme CysN/CysC Chains: A, B
Molecule details ›
Chains: A, B
Length: 173 amino acids
Theoretical weight: 19.06 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P9WNM5 (Residues: 440-612; Coverage: 28%)
Gene names: MTCY373.05, Rv1286, cysN, cysNC
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P212121
Unit cell:
a: 63.93Å b: 70.11Å c: 79.28Å
α: 90° β: 90° γ: 90°
R R work R free
0.199 0.196 0.253
Expression system: Escherichia coli BL21(DE3)