4buj

X-ray diffraction
3.7Å resolution

Crystal structure of the S. cerevisiae Ski2-3-8 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antiviral helicase SKI2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 1044 amino acids
Theoretical weight: 115.32 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P35207 (Residues: 1-834, 1086-1287; Coverage: 81%)
Gene names: L8084.17, SKI2, YLR398C
Sequence domains:
Structure domains:
Superkiller protein 3 Chains: B, F
Molecule details ›
Chains: B, F
Length: 1436 amino acids
Theoretical weight: 164.28 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P17883 (Residues: 1-1432; Coverage: 100%)
Gene names: P9677.7, SKI3, YPR189W
Sequence domains:
Antiviral protein SKI8 Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 397 amino acids
Theoretical weight: 44.28 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q02793 (Residues: 1-397; Coverage: 100%)
Gene names: REC103, SKI8, YGL213C
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 183.375Å b: 200.415Å c: 340.179Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.265
Expression system: Trichoplusia ni