4bs2

Solution NMR

NMR structure of human TDP-43 tandem RRMs in complex with UG-rich RNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
TAR DNA-binding protein 43 Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13148 (Residues: 102-269; Coverage: 41%)
Gene names: TARDBP, TDP43
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
5'-R(*GP*UP*GP*UP*GP*AP*AP*UP*GP*AP*AP*UP)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.88 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: SIMULATED ANNEALING COMBINED WITH MOLECULAR DYNAMICS IN TORSION ANGLE SPACE
Chemical shifts: BMR19290  
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided