X-ray diffraction
2.8Å resolution

Crystal structure of a human Suppressor of fused (SUFU)-GLI1p complex


Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein; Suppressor of fused homolog Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 753 amino acids
Theoretical weight: 83.63 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
  • Canonical: P0AEX9 (Residues: 27-393; Coverage: 99%)
  • Canonical: Q9UMX1 (Residues: 32-278, 358-359, 361-483; Coverage: 76%)
Gene names: JW3994, SUFU, UNQ650/PRO1280, b4034, malE
Sequence domains:
Structure domains: Sufu, C-terminal domain
Zinc finger protein GLI1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 17 amino acids
Theoretical weight: 1.65 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P08151 (Residues: 115-131; Coverage: 2%)
Gene names: GLI, GLI1

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 116.3Å b: 137.6Å c: 116.54Å
α: 90° β: 105.49° γ: 90°
R R work R free
0.198 0.197 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided