4be8

X-ray diffraction
3Å resolution

NEDD4 HECT A889F structure

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase NEDD4 Chain: A
Molecule details ›
Chain: A
Length: 385 amino acids
Theoretical weight: 45.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46934 (Residues: 938-1319; Coverage: 29%)
Gene names: KIAA0093, NEDD4, NEDD4-1, PIG53
Sequence domains: HECT-domain (ubiquitin-transferase)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 100.545Å b: 100.545Å c: 96.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.246 0.295
Expression system: Escherichia coli BL21(DE3)