4bay

X-ray diffraction
3.1Å resolution

Function and Biology Details

Reaction catalysed:
(1a) a [histone H3]-N(6),N(6)-dimethyl-L-lysine(4) + acceptor + H(2)O = a [histone H3]-N(6)-methyl-L-lysine(4) + formaldehyde + reduced acceptor
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysine-specific histone demethylase 1A Chain: A
Molecule details ›
Chain: A
Length: 686 amino acids
Theoretical weight: 76.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O60341 (Residues: 172-369, 370-852; Coverage: 80%)
  • Best match: O60341-2 (Residues: 192-876)
Gene names: AOF2, KDM1, KDM1A, KIAA0601, LSD1
Sequence domains:
Structure domains:
REST corepressor 1 Chain: B
Molecule details ›
Chain: B
Length: 133 amino acids
Theoretical weight: 15.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UKL0 (Residues: 311-443; Coverage: 27%)
Gene names: KIAA0071, RCOR, RCOR1
Sequence domains: Myb-like DNA-binding domain
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I222
Unit cell:
a: 117.98Å b: 179.12Å c: 233.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.226
Expression system: Escherichia coli BL21