PDBe 4b8a

X-ray diffraction
2.4Å resolution

Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1

Released:

Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Poly(A) ribonuclease POP2 Chain: B
Molecule details ›
Chain: B
Length: 286 amino acids
Theoretical weight: 33.15 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39008 (Residues: 151-433; Coverage: 65%)
Gene names: CAF1, N3470, POP2, YNR052C
Sequence domains: CAF1 family ribonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H
General negative regulator of transcription subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25655 (Residues: 755-1000; Coverage: 12%)
Gene names: CDC39, NOT1, ROS1, YCR093W, YCR1151, YCR93W
Sequence domains: CCR4-NOT transcription complex subunit 1 CAF1-binding domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 71.85Å b: 76.56Å c: 109.908Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.236
Expression system: Escherichia coli BL21(DE3)