4ayb

X-ray diffraction
3.2Å resolution

RNAP at 3.2Ang

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
DNA-directed RNA polymerase subunit A'' Chain: C
Molecule details ›
Chain: C
Length: 395 amino acids
Theoretical weight: 43.79 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB54 (Residues: 1-395; Coverage: 100%)
Gene names: rpo1C, rpoA2
Sequence domains: RNA polymerase Rpb1, domain 5
Structure domains: DNA polymerase; domain 1
DNA-directed RNA polymerase subunit D Chain: D
Molecule details ›
Chain: D
Length: 265 amino acids
Theoretical weight: 30.18 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB56 (Residues: 1-265; Coverage: 100%)
Gene names: rpo3, rpoD
Sequence domains:
Structure domains:
S1 motif domain-containing protein Chain: E
Molecule details ›
Chain: E
Length: 180 amino acids
Theoretical weight: 20.33 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB57 (Residues: 1-180; Coverage: 100%)
Gene name: rpo7
Sequence domains:
Structure domains:
RNA polymerase subunit 4 Chain: F
Molecule details ›
Chain: F
Length: 113 amino acids
Theoretical weight: 12.82 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB58 (Residues: 1-113; Coverage: 100%)
Gene name: rpo4
Sequence domains: RNA polymerase Rpb4
Structure domains:
RNA polymerase subunit 8 Chain: G
Molecule details ›
Chain: G
Length: 132 amino acids
Theoretical weight: 15.14 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB59 (Residues: 1-132; Coverage: 100%)
Gene name: rpo8
Sequence domains: DNA-directed RNA polymerase, subunit G
Structure domains: Nucleic acid-binding proteins
DNA-directed RNA polymerase subunit H Chain: H
Molecule details ›
Chain: H
Length: 84 amino acids
Theoretical weight: 9.69 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB60 (Residues: 1-84; Coverage: 100%)
Gene names: rpo5, rpoH
Sequence domains: RNA polymerase Rpb5, C-terminal domain
Structure domains: RPB5-like RNA polymerase subunit
DNA-directed RNA polymerase subunit K Chain: K
Molecule details ›
Chain: K
Length: 95 amino acids
Theoretical weight: 10.73 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB61 (Residues: 1-95; Coverage: 100%)
Gene names: rpo6, rpoK
Sequence domains: RNA polymerase Rpb6
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
DNA-directed RNA polymerase subunit L Chain: L
Molecule details ›
Chain: L
Length: 92 amino acids
Theoretical weight: 10.21 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB62 (Residues: 1-92; Coverage: 100%)
Gene names: rpo11, rpoL
Sequence domains: RNA polymerase Rpb3/Rpb11 dimerisation domain
Structure domains: RNA polymerase, RBP11-like subunit
DNA-directed RNA polymerase subunit N Chain: N
Molecule details ›
Chain: N
Length: 66 amino acids
Theoretical weight: 7.62 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB63 (Residues: 1-66; Coverage: 100%)
Gene names: rpo10, rpoN
Sequence domains: RNA polymerases N / 8 kDa subunit
Structure domains: Homeodomain-like
DNA-directed RNA polymerase subunit P Chain: P
Molecule details ›
Chain: P
Length: 48 amino acids
Theoretical weight: 5.61 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB64 (Residues: 1-48; Coverage: 100%)
Gene names: rpo12, rpoP
Structure domains: RNA polymerase ii, chain L
RNA_pol_Rpo13 domain-containing protein Chain: Q
Molecule details ›
Chain: Q
Length: 104 amino acids
Theoretical weight: 12.17 KDa
Source organism: Saccharolobus shibatae
UniProt:
  • Canonical: B8YB65 (Residues: 1-104; Coverage: 100%)
Gene name: rpo13
Sequence domains: RNA polymerase Rpo13 subunit HTH domain
Structure domains: DNA Excision Repair, Uvrb; Chain A

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21212
Unit cell:
a: 195.725Å b: 212.415Å c: 128.757Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.242 0.3