PDBe 4aw4

X-ray diffraction
1.93Å resolution

Engineered variant of Listeria monocytogenes InlB internalin domain with an additional leucine rich repeat inserted

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Internalin B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 311 amino acids
Theoretical weight: 34.68 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25147 (Residues: 36-98, 77-321; Coverage: 44%)
Gene names: inlB, lmo0434
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: C2
Unit cell:
a: 179.08Å b: 102.15Å c: 69.81Å
α: 90° β: 90.23° γ: 90°
R-values:
R R work R free
0.168 0.167 0.205
Expression system: Escherichia coli BL21(DE3)