› 4auu

X-ray diffraction
1.6Å resolution

Crystal structure of apo FimH lectin domain at 1.5 A resolution

Released:
DOI: 10.2210/pdb4auu/pdb
PDB 4auu coloured by chain and viewed from the front.
PDB 4auu coloured by chain and viewed from the side.
PDB 4auu coloured by chain and viewed from the top.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
The Tyrosine Gate as a Potential Entropic Lever in the Receptor-Binding Site of the Bacterial Adhesin FimH.
Wellens A, Lahmann M, Touaibia M, Vaucher J, Oscarson S, Roy R, Remaut H, Bouckaert J
Biochemistry (2012)
PMID: 22657089

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 1 fimbrin D-mannose specific adhesin Chains: A, B
Molecule details ›
Chains: A, B
Length: 158 amino acids
Theoretical weight: 16.92 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08191 (Residues: 22-179; Coverage: 57%)
Gene names: JW4283, b4320, fimH
Sequence domains: FimH, mannose binding
Structure domains: Fimbrial-type adhesion domain

Ligands and Environments

3 bound ligands:
Ligand NI 2 x NI
Ligand SO4 1 x SO4
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3121
Unit cell:
a: 91.046Å b: 91.046Å c: 79.331Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.157 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Neutralizing Antibodies Against Allosteric Proteins: Insights From a Bacterial Adhesin.
Sokurenko et al. (2022)
8 more

5 mentions without citation

Mutation of Tyr137 of the universal Escherichia coli fimbrial adhesin FimH relaxes the tyrosine gate prior to mannose binding.
Rabbani et al. (2017)
4 more