› 4ao1

X-ray diffraction
1.58Å resolution

High resolution crystal structure of bovine pancreatic ribonuclease crystallized using ionic liquid

Released:
DOI: 10.2210/pdb4ao1/pdb
PDB entry 4ao1 coloured by chain, front view.
PDB entry 4ao1 coloured by chain, side view.
PDB entry 4ao1 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Hofmeister Effects of Ionic Liquids in Protein Crystallization: Direct and Water-Mediated Interactions
Kowacz M, Mukhopadhyay A, Carvalho AL, Esperanca JMSS, Romao MJ, Rebelo LPN
CrystEngComm 14 4912- (2012)

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3221
Unit cell:
a: 64.006Å b: 64.006Å c: 63.616Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.162 0.196

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