4aim

X-ray diffraction
3.3Å resolution

Crystal structure of C. crescentus PNPase bound to RNase E recognition peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polyribonucleotide nucleotidyltransferase Chain: A
Molecule details ›
Chain: A
Length: 726 amino acids
Theoretical weight: 78.15 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AC32 (Residues: 1-712; Coverage: 100%)
Gene names: CC_0034, pnp
Sequence domains:
Structure domains:
Ribonuclease E Chain: C
Molecule details ›
Chain: C
Length: 14 amino acids
Theoretical weight: 1.8 KDa
Source organism: Caulobacter vibrioides
Expression system: Not provided
UniProt:
  • Canonical: Q9A749 (Residues: 885-898; Coverage: 2%)
Gene names: CC_1877, rne

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P63
Unit cell:
a: 97.329Å b: 97.329Å c: 191.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.186 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided