PDBe 4af3

X-ray diffraction
2.75Å resolution

Human Aurora B Kinase in complex with INCENP and VX-680

Released:
Source organism: Homo sapiens
Primary publication:
Crystal Structure of Human Aurora B in Complex with INCENP and VX-680.
OpenAccess logo J. Med. Chem. 55 7841-8 (2012)
PMID: 22920039

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aurora kinase B Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96GD4 (Residues: 55-344; Coverage: 84%)
Gene names: AIK2, AIM1, AIRK2, ARK2, AURKB, STK1, STK12, STK5
Sequence domains: Protein kinase domain
Structure domains:
Inner centromere protein Chain: D
Molecule details ›
Chain: D
Length: 71 amino acids
Theoretical weight: 8.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NQS7 (Residues: 835-903; Coverage: 8%)
Gene name: INCENP

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3121
Unit cell:
a: 80.145Å b: 80.145Å c: 92.542Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.205 0.264
Expression system: Escherichia coli BL21(DE3)