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Electron Microscopy
13.5Å resolution

Structure of the E. coli methyltransferase KsgA bound to the E. coli 30S ribosomal subunit

Released:
Source organism: Escherichia coli
Related structures: EMD-2017

Function and Biology Details

Reaction catalysed:
4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA 
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
Entry contents:
21 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (22 distinct):
30S ribosomal protein S2 Chain: B
Molecule details ›
Chain: B
Length: 240 amino acids
Theoretical weight: 26.65 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 2-241; Coverage: 100%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
30S ribosomal protein S3 Chain: C
Molecule details ›
Chain: C
Length: 232 amino acids
Theoretical weight: 25.9 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 2-233; Coverage: 100%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
30S ribosomal protein S4 Chain: D
Molecule details ›
Chain: D
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 2-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
30S ribosomal protein S5 Chain: E
Molecule details ›
Chain: E
Length: 166 amino acids
Theoretical weight: 17.5 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 2-167; Coverage: 99%)
Gene names: JW3265, b3303, rpsE, spc
Sequence domains:
30S ribosomal protein S6 Chain: F
Molecule details ›
Chain: F
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-135; Coverage: 100%)
Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6
30S ribosomal protein S7 Chain: G
Molecule details ›
Chain: G
Length: 178 amino acids
Theoretical weight: 19.92 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02359 (Residues: 2-179; Coverage: 99%)
Gene names: JW3303, b3341, rpsG
30S ribosomal protein S8 Chain: H
Molecule details ›
Chain: H
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 2-130; Coverage: 99%)
Gene names: JW3268, b3306, rpsH
Sequence domains: Ribosomal protein S8
30S ribosomal protein S9 Chain: I
Molecule details ›
Chain: I
Length: 129 amino acids
Theoretical weight: 14.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7X3 (Residues: 2-130; Coverage: 99%)
Gene names: JW3199, b3230, rpsI
Sequence domains: Ribosomal protein S9/S16
30S ribosomal protein S10 Chain: J
Molecule details ›
Chain: J
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R5 (Residues: 1-103; Coverage: 100%)
Gene names: JW3283, b3321, nusE, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
30S ribosomal protein S11 Chain: K
Molecule details ›
Chain: K
Length: 128 amino acids
Theoretical weight: 13.74 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 2-129; Coverage: 99%)
Gene names: JW3259, b3297, rpsK
Sequence domains: Ribosomal protein S11
30S ribosomal protein S12 Chain: L
Molecule details ›
Chain: L
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 2-124; Coverage: 99%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
30S ribosomal protein S13 Chain: M
Molecule details ›
Chain: M
Length: 117 amino acids
Theoretical weight: 13 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S9 (Residues: 2-118; Coverage: 99%)
Gene names: JW3260, b3298, rpsM
Sequence domains: Ribosomal protein S13/S18
30S ribosomal protein S14 Chain: N
Molecule details ›
Chain: N
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 2-101; Coverage: 99%)
Gene names: JW3269, b3307, rpsN
Sequence domains: Ribosomal protein S14p/S29e
30S ribosomal protein S15 Chain: O
Molecule details ›
Chain: O
Length: 89 amino acids
Theoretical weight: 10.32 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
30S ribosomal protein S16 Chain: P
Molecule details ›
Chain: P
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Sequence domains: Ribosomal protein S16
30S ribosomal protein S17 Chain: Q
Molecule details ›
Chain: Q
Length: 83 amino acids
Theoretical weight: 9.59 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 2-84; Coverage: 99%)
Gene names: JW3273, b3311, neaA, rpsQ
Sequence domains: Ribosomal protein S17
30S ribosomal protein S18 Chain: R
Molecule details ›
Chain: R
Length: 74 amino acids
Theoretical weight: 8.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 2-75; Coverage: 99%)
Gene names: JW4160, b4202, rpsR
Sequence domains: Ribosomal protein S18
30S ribosomal protein S19 Chain: S
Molecule details ›
Chain: S
Length: 91 amino acids
Theoretical weight: 10.32 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U3 (Residues: 2-92; Coverage: 99%)
Gene names: JW3278, b3316, rpsS
Sequence domains: Ribosomal protein S19
30S ribosomal protein S20 Chain: T
Molecule details ›
Chain: T
Length: 86 amino acids
Theoretical weight: 9.58 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 2-87; Coverage: 99%)
Gene names: JW0022, b0023, rpsT
Sequence domains: Ribosomal protein S20
30S ribosomal protein S21 Chain: U
Molecule details ›
Chain: U
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 1-71; Coverage: 100%)
Gene names: JW3037, b3065, rpsU
Sequence domains: Ribosomal protein S21
Ribosomal RNA small subunit methyltransferase A Chain: V
Molecule details ›
Chain: V
Length: 252 amino acids
Theoretical weight: 27.99 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P06992 (Residues: 17-268; Coverage: 92%)
Gene names: JW0050, b0051, ksgA, rsmA
16S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1542 nucleotides
Theoretical weight: 499.69 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 13.5Å
Relevant EMDB volumes: EMD-2017
Expression system: Escherichia coli K-12