4acy

X-ray diffraction
1.69Å resolution

Selenomethionine derivative of the GH99 endo-alpha-mannosidase from Bacteroides thetaiotaomicron

Released:

Function and Biology Details

Reaction catalysed:
GlcMan(9)GlcNAc(2)-[protein] + H(2)O = Man(8)GlcNAc(2)-[protein] (isomer 8A(1,2,3)B(1,2)) + alpha-D-glucosyl-(1->3)-alpha-D-mannopyranose
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-alpha-mannosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 43.64 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8A109 (Residues: 1-382; Coverage: 100%)
Gene name: BT_3862
Sequence domains: Glycosyl hydrolase family 99
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P1
Unit cell:
a: 47.721Å b: 60.002Å c: 78.666Å
α: 103.45° β: 102.59° γ: 98.59°
R-values:
R R work R free
0.177 0.175 0.208
Expression system: Escherichia coli BL21