4a5x

X-ray diffraction
1.91Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MIT domain-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 86 amino acids
Theoretical weight: 10.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8WV92 (Residues: 9-85; Coverage: 31%)
Gene name: MITD1
Sequence domains: MIT (microtubule interacting and transport) domain
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit
Charged multivesicular body protein 1a Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 amino acids
Theoretical weight: 1.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HD42 (Residues: 184-196; Coverage: 7%)
Gene names: CHMP1, CHMP1A, KIAA0047, PCOLN3, PRSM1

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 67.07Å b: 74.16Å c: 89.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.226
Expression system: Escherichia coli BL21