4a1g

X-ray diffraction
2.6Å resolution

The crystal structure of the human Bub1 TPR domain in complex with the KI motif of Knl1

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic checkpoint serine/threonine-protein kinase BUB1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 18.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43683 (Residues: 1-150; Coverage: 14%)
Gene names: BUB1, BUB1L
Sequence domains: Mad3/BUB1 homology region 1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Kinetochore scaffold 1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 53 amino acids
Theoretical weight: 5.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8NG31 (Residues: 176-226; Coverage: 2%)
Gene names: CASC5, KIAA1570, KNL1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 59.279Å b: 130.969Å c: 74.956Å
α: 90° β: 110.17° γ: 90°
R-values:
R R work R free
0.189 0.186 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli BL21