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X-ray diffraction
1.94Å resolution

CRYSTAL STRUCTURE OF PA-IL LECTIN COMPLEXED WITH NPG AT 1.9 A RESOLUTION

Released:
DOI: 10.2210/pdb3zyf/pdb
PDB 3zyf coloured by chain and viewed from the front.
PDB 3zyf coloured by chain and viewed from the side.
PDB 3zyf coloured by chain and viewed from the top.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Kadam RU, Bergmann M, Hurley M, Garg D, Cacciarini M, Swiderska MA, Nativi C, Sattler M, Smyth AR, Williams P, Camara M, Stocker A, Darbre T, Reymond J-L

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PA-I galactophilic lectin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 122 amino acids
Theoretical weight: 12.9 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05097 (Residues: 1-122; Coverage: 100%)
Gene names: PA2570, lecA, pa1L
Sequence domains: PA-IL-like protein
Structure domains: Galactose-binding lectin

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand 147 4 x 147
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 84.562Å b: 84.562Å c: 163.709Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.21
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

Microfluidics Technology for the Design and Formulation of Nanomedicines.
Jaradat et al. (2021)
7 more

3 mentions without citation

Accurate macromolecular crystallographic refinement: incorporation of the linear scaling, semiempirical quantum-mechanics program DivCon into the PHENIX refinement package.
Borbulevych et al. (2014)
2 more