X-ray diffraction
1.74Å resolution

Crystal structure of POFUT1 apo-form (crystal-form-I)


Function and Biology Details

Reaction catalysed:
GDP-beta-L-fucose + [protein]-(L-serine/L-threonine) = GDP + [protein]-3-O-(alpha-L-fucosyl)-(L-serine/L-threonine)
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
GDP-fucose protein O-fucosyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 41.01 KDa
Source organism: Caenorhabditis elegans
Expression system: Komagataella pastoris
  • Canonical: Q18014 (Residues: 26-383; Coverage: 97%)
Gene name: C15C7.7
Sequence domains: GDP-fucose protein O-fucosyltransferase
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 117.44Å b: 42.533Å c: 96.924Å
α: 90° β: 107.24° γ: 90°
R R work R free
0.235 0.235 0.252
Expression system: Komagataella pastoris