› 3zxg

X-ray diffraction
3.12Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysenin Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 34.85 KDa
Source organism: Eisenia fetida
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O18423 (Residues: 2-297; Coverage: 100%)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand 0SM 1 x 0SM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 211.15Å b: 37.2Å c: 96.74Å
α: 90° β: 104.03° γ: 90°
R-values:
R R work R free
0.24 0.239 0.271
Expression system: Escherichia coli BL21

Quick links

Citations

20 review citations

Animal secretory endolysosome channel discovery.
Zhang et al. (2021)
19 more

3 mentions without citation

Crystal structure of an invertebrate cytolysin pore reveals unique properties and mechanism of assembly.
Podobnik et al. (2016)
2 more