3zw9

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 1 (RPMFE1) COMPLEXED WITH (2S,3S)-3-HYDROXY-2-METHYLBUTANOYL-COA

Released:

Function and Biology Details

Reactions catalysed:
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisomal bifunctional enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 742 amino acids
Theoretical weight: 80.93 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07896 (Residues: 1-722; Coverage: 100%)
Gene names: Ehhadh, Mfe1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 65.592Å b: 126.652Å c: 225.432Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.287
Expression system: Escherichia coli BL21(DE3)