› 3zw0

X-ray diffraction
1.6Å resolution

Structure of BambL lectin from Burkholderia ambifaria

Released:
DOI: 10.2210/pdb3zw0/pdb
PDB 3zw0 coloured by chain and viewed from the front.
PDB 3zw0 coloured by chain and viewed from the side.
PDB 3zw0 coloured by chain and viewed from the top.
Source organism: Burkholderia ambifaria AMMD
Entry authors: Audfray A, Claudinon J, Abounit S, Ruvoen-Clouet N, Larson G, Wimmerova M, LePendu J, Romer W, Varrot A, Imberty A

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fucose-binding lectin protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 87 amino acids
Theoretical weight: 9.39 KDa
Source organism: Burkholderia ambifaria AMMD
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0B4G1 (Residues: 1-87; Coverage: 100%)
Gene names: Bamb_5415, CH72_5454, rsl
Sequence domains: Fungal fucose-specific lectin
Structure domains: Lipocalin
Fucose-binding lectin protein Chain: B
Molecule details ›
Chain: B
Length: 87 amino acids
Theoretical weight: 9.4 KDa
Source organism: Burkholderia ambifaria AMMD
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0B4G1 (Residues: 1-87; Coverage: 100%)
Gene names: Bamb_5415, CH72_5454, rsl
Sequence domains: Fungal fucose-specific lectin
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand FUC 1 x FUC
1 modified residue:
Ligand MHO 1 x MHO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 45.11Å b: 47.25Å c: 98.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.143 0.188
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Grafting Dendrons onto Pillar[5]Arene Scaffolds.
Nierengarten et al. (2021)
5 more

2 mentions without citation

Fucose-binding lectin from opportunistic pathogen Burkholderia ambifaria binds to both plant and human oligosaccharidic epitopes.
Audfray et al. (2012)
1 more